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Sonea is called to the Magicians’ Guild. After a period of instability following the defeat of the Guild’s mage. Noire is struck by a new disease and Fever sets her plan to work in reverse. Sonea and Lorkin fight to save the magicians’ guild from the dark forces that stand between them and the .
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Omnipotent: A Watchful Eye on the Astronomical Logos Trilogy Book 1. From The Novice to the High Lord of the Magicians’ Guild. (Trudi Canavan) From the high Queen’s city to the depths of the Underworld, Sonea takes a journey towards Her power and knowledge and her mission is to. The Labyrinthine Mind: A Watchful Eye on the Astronomical Logos Trilogy Book 2.
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The Magicians’ Guild, The Novice. Sonea is called to the Magicians’ Guild. As she enters this sixth book in Trudi Canavan’s Black Magician trilogy, she is a naive novice, with not.
The High Lord The Black Magician Trilogy Book 3. Discover the magic of Trudi Canavan with her brand new novel in the Traitor Spy trilogy. When word comes that Lorkin has gone missing, Sonea is desperate to.
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Ketol acid reductoisomerase (KARI) catalyzes the formation of 3-methyl-2-oxobutyric acid from L-2-keto-3-methylglutarate in Proteus mirabilis. A gene encoding the enzyme was isolated from P. mirabilis chromosomal DNA by polymerase chain reaction (PCR) amplification using oligonucleotide primers corresponding to sequences at the 5′- and 3′-ends of a gene from Yarrowia lipolytica (KF-KAR). The recombinant KAR gene was overexpressed in Escherichia coli. The KAR protein was purified to homogeneity, and the enzyme was characterized. KARI has a molecular weight of 64 kDa and is comprised of four identical subunits with a molecular mass of 32 kDa. The enzyme utilizes 3-methyl-2-oxobutyric acid as a substrate in the production of 3-methyl-2-butenoic acid. The optimum pH is 7.5, and the enzyme is stable between pH 7.0 and 8.0. The K(m) values for 3-methyl-2-oxobutyric acid and NADH are 0.037 and 0.047 mM, respectively. The K(m) for 3-methyl-2-oxobutyric acid is reduced by 10- to
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