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Coronal sections of the rat hippocampus were studied in serial steps for the pattern of cellular injury after excitotoxic lesions of the entorhinal cortex. Previous work has shown that nerve cells degenerate in the dentate gyrus of the hippocampus after entorhinal excitotoxic lesions. In this study, the temporal course of degeneration in the dentate gyrus was followed using different probes of the cell damage. The intraparenchymal retrograde transport of cholera toxin subunit B (CTXB), the presence of necrotic neurons in the dentate gyrus, and the presence of acid phosphatase-positive cells (AcPase+) demonstrated that the cellular damage begins in the granule cells of the dentate gyrus, with subsequent degeneration of CA4 pyramidal neurons and CA3 neurons. In contrast, examination of the degenerating CA1 and CA2 neurons using Cresyl violet staining showed that the first changes to occur in those neurons are the reactive glial changes. Immunohistochemical staining demonstrated that astrocytes are among the first cells in the dentate gyrus and hilus of the hippocampus to be altered by the excitotoxic injury. On the basis of the results presented in this paper, it is clear that much of the initial cellular damage in the hippocampus after entorhinal excitotoxic lesions is carried out by the astrocytes and possibly also by the oligodendrocytes and by the neurons of the entorhinal cortex.Magellan Passport 3
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