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GenBank-Submit only. A very useful application. EMBL-Submit only. A very useful application. DDBJ-Submit only. A very useful application. Phylogenetics-Submit only. A very useful application. Segmented – Submit only. A very useful application. Cross-Submit (EMBL-DDBJ/GenBank). Submit only. Submission of smaller elements in sequence submissions such as a segment of a plasmid or clone might be handled as if it was a single sequence. Segmented Submission: Sequin Free Download contains a module for the submission of sequences as a collection of smaller sequences that together form a single sequence. It supports submissions that include up to seven sequences. Sequence Handling in Sequin: Very small sequences such as a single contig can be submitted in a Sequin submission. A Sequin entry can only handle sequences that are shorter than 2550 characters. Protein sequences longer than 250 amino acids are submitted to GenBank or EMBL as FASTA format. Sequences shorter than 250 amino acids are submitted as a Sequin sequence. Protein sequences are submitted to EMBL as FASTA format. If no model number is associated with the protein, the entries in the Protein data field are highlighted and a red box is underlined. The sequence data entry is submitted as FASTA format. The Nucleotide sequence of a gene can be submitted as DNA or a Sequin sequence, depending on the length of the sequence. A Sequin entry is submitted to GenBank as two separate sequences; the first is submitted as a Sequin sequence and the second is submitted as a DNA sequence (FASTA format). The first sequence of a Sequin segmented submission must always be a Sequin sequence. The first Sequin sequence is submitted as a Sequin sequence. The second sequence is a Sequin sequence. The Sequin sequence includes the GenBank or EMBL sequence identifier and the accession number of the Sequin sequence. The third and subsequent Sequin sequences are submitted as DNA sequences (FASTA format) and the sequence identifier of each sequence is recorded. The DDBJ submission is submitted as a Sequin sequence. The sequence identifier of a Sequin submission is the same as that used in the EMBL submission. Sub
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This is a GUI application designed for Windows and a Graphical User Interface for Unix. The application is split into several distinct, yet related, components. The components are: – Sequin: The core of the application – A front end for the submission, updating, and distribution of sequences and other data via the GenBank, EMBL and DDBJ sequence database. – SequinPrefs: The Sequin preferences dialog – a collection of standard parameters for Sequin that allow the user to customize Sequin to their needs. – SequinDB: The database source for Sequin – SequinDB is independent of, and currently compatible with, the UniGene gene index of the National Center for Biotechnology Information (NCBI) – SequinFrontend: Sequin’s graphical user interface – A collection of all the components of Sequin including dialog boxes, lists, drop down menus, and other user interfaces required to submit data to the database. – SequinApplet: The applet that hosts Sequin – An applet (programs that can be embedded on a web page) that hosts the graphical user interface, the preferences, and other administrative data needed to customize Sequin to the user’s needs. – SequinDocGen: Sequin’s generation and distribution of documentation files – SequinDocGen is a tool that generates and updates DocGen files for Sequin. Sequin can be used as either a stand-alone program or as an application within the Bio-Seq application. See the Software Index to see how Sequin fits into the bioinformatics world of sequence biology. Download: The latest version of Sequin and its associated documentation is available free from the W3C Sequin website. Download Sequin 1.2: Sequin 1.2: The Sequin 1.2 update package includes the following components: – Sequin-1.2: A stand-alone version of the Sequin application. – SequinPrefs-1.2: The SequinPrefs application. – SequinDocGen-1.2: The SequinDocGen application. – SequinFrontend-1.2: The SequinFrontend application. – SequinApplet-1.2: The SequinApplet application. – Sequin:1.2.zip: b7e8fdf5c8
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This is a detailed manual for Sequencher 4.10.1, which includes : Overview of the Sequencher program and its features Introductory sequencing project Configuration of data analysis in Sequencher 4.10.1 Detailed step-by-step instructions for creating a new submission project in Sequencher Creating your first submission project in Sequencher Creating a basic project for a single mRNA, cDNA, or EST sequence Submitting sequences to the GenBank, EMBL, or DDBJ databases Submitting sequences to the EMBL or DDBJ databases Submitting sequences to the GenBank, EMBL, or DDBJ databases with dbSNP accession numbers or HUGO gene symbols Submitting sequences to the GenBank, EMBL, or DDBJ databases with accession numbers Creating your first project in Sequencher Sequin Overview: Sequin lets you create a new submission project for a single sequence, cDNA, EST, or multiple sequences. Enter the nucleotide or protein sequence into the sample space, select the sequence type and the file format, and hit the submit button. A submission project in Sequencher contains information about the sequence and its associated attributes, such as the sequence name and the source organism. Sequencher then creates a submission record in your submission database and initiates the process of submitting the sequence to the appropriate EMBL or GenBank database. In your submission project, you can select from a menu of submission options to permit users or automated software to process and submit your sequences. You can also specify a unique accession number, assign sequence types or sequence formats, and choose a serial identifier. Table 1. Sequencher Project Configuration Features: Feature Description Format The format you chose for storing your sequences in the input file. Sequencher will retain the format from your input file for this feature. Format choices are: DNAsingleLetter-A.ai | DNAsingleLetter-C.ai DNAsingleLetter-D.ai | DNAsingleLetter-T.ai DNAsingleLetter-G.ai | DNAsingleLetter-N.ai DNAsingleLetter-P.ai | DNAsingleLetter-Q.ai DNAsingleLetter-R.ai | DNAsingleLetter-S
What’s New in the?
================== * Strong focus on ease of use — the program is designed to be used by anyone, even one with little or no computer experience. * Powerful, easy-to-use graphing facilities. * Very fast for long submissions. * Supports fast standard connection and local network file transfers. * Supports large local directories and file transfer. * Freezes the program and Internet browser at the point of the last transmission, which makes debugging easier. * Extensive documentation is included. * Extensive script file option which supports advanced format. If your data is ready to submit, or you just want to update a submitted entry, you’re in the right place. Sequin has two main interfaces for submitting sequences. There is a command line interface, which gives complete control over the submission process. The program also has an optional graphical display and user interface which can be used for streamlined submissions. Installation Information ======================== * Simply open a DOS command window and type “sequin” at the prompt. * Choose the Install option, then choose the desired install directory. You can specify installation directories for the program and its documentation, or you can choose to install them at the default location. * Type “sequin –help” at the DOS prompt to view the appropriate commands. * To uninstall the program (if previously installed), simply run “sequin -x”. How to Use Sequin ================= * The command line interface is the primary way to submit and update entries to the GenBank, EMBL, or DDBJ sequence databases. * For ordinary submissions, type “sequin submit” at the command line. * For complex submissions, type “sequin sequence” followed by a single sequence name to submit a single sequence. * Type “sequin submit –help” for information about the full set of available options. * The program is set up to accept long submissions from the command line. This means that your sequence file, or files, will be broken into three or more “chunks” for submitting. Each chunk is named sequentially in the order in which they are to be submitted. The maximum chunk size is 2,048 bytes. * You can turn on the graphical interface and use it as a front end for the command line submission. The graphical interface is only supported for sequence submissions. The command line interface is available at all times
System Requirements:
MP1-SE (Mega Personalities 1 – Enhanced): MP2-SE (Mega Personalities 2 – Enhanced): MP3-SE (Mega Personalities 3 – Enhanced): MP4-SE (Mega Personalities 4 – Enhanced): MP5-SE (Mega Personalities 5 – Enhanced): Publisher: 80s N’ Go Game Type: Fighting Games – Tag Team Game Version: MP2-SE (2002-2003) Developer: 80s
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